Table 4.

PCR reaction solution.

Reagent components	Volume	Final concentration
2X qPCR MasterMix	10 μl	1X
Forward primer (10 μM)	0.6 μl	300 nM
Reverse primer (10 μM)	0.6 μl	300 nM
cDNA (2 μg)	0.4 μl
Nuclease-Free Water	Up to 20 μl