Table 5.
PCR amplification conditions.
| Steps | Number of cycles | Temperature | Time | Detection |
|---|---|---|---|---|
| Predenaturation | 1 | 95°C | 10 min | Off |
| Denaturation | 40 | 95°C | 5 sec | Off |
| Annealing and extension | 60°C | 30 min | On |
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