Table 6.

Proportion of separating glue.

Name of reagents	Volume
DDW	4.825 mL
30% acrylamide	2.475 mL
1.5 M Tris HCl (pH 8.8)	2.5 mL
10% SDS	100 μL
10% ammonium persulfate	100 μL
TEMED	4 μL