Table 7.
Proportion of concentrated adhesive.
| Name of reagents | Volume |
|---|---|
| DDW | 2.225 mL |
| 30% acrylamide | 375 μL |
| 1.0 M Tris HCl (pH 6.8) | 380 μL |
| 10% SDS | 30 μL |
| 10% ammonium persulfate | 30 μL |
| TEMED | 3 μL |
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