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3D reconstruction of mitochondrial structures in D. magnusii cells. Exponentially grown yeast cells were incubated in (a) fresh portion of growth medium or (b) with additionally added 45 μM BAC for 1 h. Then, cells were washed with 50 mM PBS, pH 5.5, and incubated with 200 nM MitoTracker Green for 30 min. The full video is presented in Video 3 in the Supplementary Material. Scale bars are 10 μm.
