Figure 2.

Effect of CCCP and varying BAC concentrations on state 4 respiration (V) of rat liver mitochondria respiring on (a) succinate or (b) glutamate+malate. First derivatives of amperometric curves are shown. The basic incubation medium was supplemented with 0.5 mM EGTA, mitochondria (0.5 mg protein/ml), and either (a) 20 mM Tris-succinate+rotenone (2 μg/mg protein) or (b) 20 mM Tris-glutamate and 5 mM Tris-malate. Additions and amounts are given on the figures. Arrows point to the addition of CCCP and varying BAC concentrations. V₀ is defined as respiratory rate in state 4 respiration before the addition of CCCP and varying BAC concentrations (24.8 or 11.2 ng-atom O/min per mg protein upon oxidation of succinate or glutamate+malate, respectively).