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Membrane depolarization by BAC. The basic incubation medium was supplemented with 0.5 mM EGTA, 20 mM safranin O, mitochondria (0.5 mg protein/ml), and either 20 mM Tris-glutamate and 5 mM Tris-malate (curve 1) or 20 mM Tris-succinate+rotenone (2 μg/mg protein) (curve 2).
