Figure 6.

Inhibitory effect of BAC on ATP production by rat liver mitochondria respiring on succinate. The basal incubation medium was supplemented with 0.5 mM EGTA, 6 μM Ap5A, an inhibitor of adenylate kinase, 20 mM Tris-succinate+rotenone (2 μg/mg protein), and either (a) 25 μM Phenol red and mitochondria (0.5 mg protein/ml) or (b) 1 mM glucose, 1 mM NADP, 10 U/ml hexokinase, 3 U/ml glucose-6-phosphate dehydrogenase, and mitochondria (0.25 mg protein/ml). Where indicated, oligomycin (Oligo, 1 μg/mg protein) and varying BAC concentrations were added. The statistical analyses were carried out by the one-way ANOVA test. ^∗∗∗p < 0.001; ^∗∗0.001 < p < 0.01.