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Hydrogen peroxide generation by rat liver mitochondria respiring on succinate. The basal incubation medium was supplemented with 0.5 mM EGTA, 20 mM Tris-succinate, 6 mM aminotriazole, 5 μM Amplex Red, horseradish peroxidase (9 IU/ml), and mitochondria (0.25 mg protein/ml). The statistical analyses were carried out by the one-way ANOVA test against control. ∗∗0.001 < p < 0.01; ∗∗∗∗p < 0.0001.
