Figure 8.

BAC-induced oxidative stress in the Y. lipolytica yeast. Exponentially grown yeast cells were either incubated with 10 μM BAC for 2 h (b, marked by black) or initially preincubated with 3 μM SkQ1 for 30 min and then incubated with 10 μM BAC for 2 h (b, marked by light grey). All samples were rinsed with a fresh portion of growth medium and incubated with 15 μM H₂DCF-DA for 30 min in the dark. Stained cells were analyzed by flow cytometry. The statistical analyses were carried out by the one-way ANOVA test. ^∗∗0.001 < p < 0.01; ^∗0.01 < p < 0.05.