Figure 9.

BAC-triggered mitochondria fragmentation in D. magnusii cells, the protective effect of SkQ1. Exponentially grown yeast cells were used. (a) Control cells; (b) cells were incubated with 800 nM SkQ1 for 1 h; (c) cells were incubated with 45 μM BAC for 1 h; (d) cells were preincubated with 800 nM SkQ1 for 1 h, washed in 50 mM PBS buffer, pH 5.5, suspended in a fresh portion of growth medium and then incubated with 45 μM BAC for 1 h. For visualization of mitochondria, cells were washed with 50 mM PBS, pH 5.5, and stained with 200 nM MitoTracker Green for 30 min. Scale bars are 10 μm.