Skip to main content
. 2020 Feb 5;117(7):3848–3857. doi: 10.1073/pnas.1918215117

Fig. 3.

Fig. 3.

NAS requires IDO1 expression to activate AhR. (A) AhR transactivation activity by Kyn and NAS in IDO1−/− H1L1 hepatoma cells. Data (mean ± SD from 3 experiments in triplicate) are presented as fold change in normalized transactivation activity in cells incubated with Trp metabolites relative to medium alone (in which fold change =1; dotted line). (B) Real-time PCR analysis of Cyp1a1 transcripts in WT and Ido1−/− cDCs incubated with Kyn or NAS at different concentrations for 12 h, using Gapdh for normalization. Data are presented as in A. **P < 0.01, ***P < 0.001. One representative experiment of 3 is shown.