Fig. 3.

Temporal assessment of canine subclinical detection of CLas infection (black) compared with qPCR detection/confirmation at two accepted regulatory qPCR thresholds for 30 psyllid-inoculated Valencia orange trees; 4 to 10 canines assayed each tree on each assay date (resulting in 0 to 10 detections indicated by variance bars). One sample for qPCR assay was selected from each tree consisting of four leaves split into two samples of two leaves; each was processed via qPCR (any positive subsample denoted a positive qPCR detection). CT, or cycle threshold, is the number of PCR cycles required for the fluorescent signal to cross the threshold and exceed background level and thereby denote a positive/negative assay.