Fig. 2.

Drosophila YPS preferentially bind to m⁵C-containing RNAs in germ cells. (A–H) Germ line-specific Flag-YPS and anti-m⁵C antibody pull down mostly overlapped total RNAs, rRNAs, tRNAs, LncRNAs, premiRNAs, asRNAs, snoRNAs, snRNAs, and mRNAs (numbers represent different RNAs). (I and J′) YPS-binding sites are closely correlated with m5C sites in mad and eIF4A mRNAs (I and I′) and in mei-P26 and sxl mRNAs (J and J″). (K and L) m⁵C sites and YPS-binding sites are more prevalent in the CDS and intron than in the 5′ UTR and the 3′ UTR. (M) Top 15 significantly overrepresented pathways for the mRNAs sharing YPS-binding sites and m5C sites based on KEGG pathway analysis.