Fig. 3.
The CSDs of Drosophila YPS and human YBX1 preferentially bind to the m5C-containing RNA. (A and B) ITC analysis of Drosophila YPS binding to m5C-containing and RNA oligos. (C and D) ITC analysis of human YBX1 binding to m5C-containing and RNA oligos. (E and F) Overall structure of the human YBX1 CSD domain in complex with to m5C-containing and RNA oligos. The m5C RNA is shown in sticks, while the YBX1 CSD is shown in cartoon. The aromatic residues that interact with the RNA bases are indicated. (G) Detailed presentation of the interactions between YBX1 CSD domain aromatic residues and individual m5C RNA bases. The residues in the CSD domain and the m5C RNA bases are shown in cyan and orange, respectively.