Fig. 1.

TIP5 plays a major role in luminal prostate cells. (A) TIP5 is required for prostate epithelium maintenance. H&E and IF staining of basal K5 and luminal K8 of the mouse ventral prostate from 6-mo-old WT and Tip5-KO mice. (Scale bars represent 200 μm [H&E] and 50 μm [IF].) (B) Representative FACS plots of CD49f+/CD24-low (basal) and CD49f-low/CD24+ (luminal) stained murine prostate cells. (C, Top) Integrative Genomics Viewer (IGV) browser view of RNAseq reads mapped at K5 and Pbsn loci in basal and luminal cells. (C, Bottom) Quantitative (q)RT-PCR expression analysis of K5 and Pbsn in isolated basal and luminal cells. Expression was normalized to Gapdh. Average expression value of K5 (three experiments) and Pbsn (two experiments). Error bars represent s.d. (D) TIP5 is expressed in both prostate luminal and basal cells. (D, Top) IGV view of RNAseq reads mapped at the Tip5 locus. (D, Bottom) qRT-PCR analysis of Tip5 expression. Values were normalized to Gapdh mRNA levels. Average value of three experiments. Error bars represent s.d. (E) TIP5 regulates gene expression in prostate luminal cells. MA plots of RNAseq data showing differentially expressed genes among luminal and basal WT and Tip5-KO cells.