Fig. 2.

The dopamine D1-D2 heteromer mediates sex differences in pro-depressive responses in the forced swim test (FST). a In FST, no sex differences was observed for the total immobility time of the test. b Time-course of FST for male and female rats. Analysis showed a significant difference in immobility time only for the first minute of the test (**p<0.01). c Vehicle-treated female rats exhibited increased immobility in the first minute of testing compared to male rats (*p < 0.05). This difference was abolished by pre-treatment with TAT-D1 peptide (**p < 0.01). d The latency, in the first minute of FST, was highly reduced in vehicle-treated female rats compared to male rats, which was abolished by TAT-D1 (*p < 0.05). Error bars represent means ± S.E.M. *p < 0.05, **p < 0.01, ANOVA followed by Bonferroni’s post-hoc. N = 6 rats/group