Table 1.
Immunoproteomic and mass-spectrometric identification of Eimeria acervulina sporozoite proteins recognized by immune sera of the three Eimeria species studied
| Spot IDa | Identified protein grouped by functions | Accession numberb | No. of matched peptidesc | Theoretical Mr/pI | Protein scored | Protein sequence coverage (%)e |
|---|---|---|---|---|---|---|
| Protein translation | ||||||
| 1 | Elongation factor 1-beta, putative, partial | XP_013248731.1 | 16/40 | 19289/4.51 | 209 | 62 |
| 6 | Eukaryotic translation initiation factor 3 subunit 8, putative | XP_013250573.1 | 14/37 | 31924/9.76 | 172 | 55 |
| Transcription | ||||||
| 8 | Elongin c, putative | XP_013249709.1 | 11/47 | 17120/5.34 | 144 | 49 |
| Cargo transport and protein trafficking | ||||||
| 2 | Intraflagellar transport particle protein, putative, partial | XP_013246990.1 | 16/46 | 28884/8.35 | 220 | 43 |
| 9 | AP-4 complex subunit sigma-1, putative | XP_013248843.1 | 11/33 | 17189/5.41 | 135 | 40 |
| Ribosome-related proteins | ||||||
| 7 | 60S ribosomal protein L8, putative | XP_013249139.1 | 12/40 | 28042/10.71 | 120 | 37 |
| 18 | Ribosome biogenesis protein brix, putative | XP_013248150.1 | 17/54 | 54150/9.82 | 170 | 38 |
| Predicted domain-containing proteins | ||||||
| 3 | Uncharacterized ACR, YagE family COG1723 domain-containing protein, putative | XP_013250980.1 | 18/45 | 20048/9.58 | 240 | 49 |
| 4 | U1 like C2H2 zinc finger, related | XP_013248237.1 | 14/35 | 28894/9.60 | 199 | 68 |
| Chaperone proteins | ||||||
| 17 | DnaJ domain-containing protein, putative | XP_013253345.1 | 15/49 | 48311/6.56 | 140 | 36 |
| Protein of enzymatic properties | ||||||
| 5 | Nucleolar GTP-binding protein, putative | XP_013252320.1 | 11/41 | 21119/9.70 | 147 | 57 |
| 10 | Glycerol-3-phosphate dehydrogenase, putative, partial | XP_013248247.1 | 17/31 | 35465/6.67 | 237 | 60 |
| 11 | Protein phosphatase 2C, putative | XP_013248554.1 | 13/56 | 42702/5.82 | 116 | 28 |
| 12 | Glyceraldehyde-3-phosphate dehydrogenase, putative | XP_013251305.1 | 16/46 | 36753/7.57 | 211 | 41 |
| 13 | Cysteine desulfurase, putative | XP_013251976.1 | 17/34 | 63362/8.43 | 191 | 45 |
| 14 | Glyoxalase, putative | XP_013247421.1 | 20/39 | 65222/6.63 | 246 | 37 |
| 15 | Phosphatase, putative | XP_013251081.1 | 14/38 | 62536/9.36 | 158 | 34 |
| 16 | Phosphoribosylpyrophosphate synthetase, putative | XP_013248652.1 | 17/40 | 47500/9.30 | 171 | 57 |
| 19 | CAM kinase, CDPK family TgPK1, putative | XP_013250395.1 | 15/36 | 118100/7.12 | 134 | 16 |
| 20 | DNA photolyase, putative | XP_013251340.1 | 14/31 | 52839/8.79 | 147 | 37 |
| 21 | gamma-glutamyl phosphate reductase, putative | XP_013252670.1 | 12/34 | 44087/5.46 | 153 | 36 |
aProtein spot numbering in the 2-DE gel; spots 22 and 23 that had no detectable protein signals are not listed
bAccession number in NCBI
cNumber of peptides that match the predicted protein sequence
dProtein score is − 10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant (P < 0.05)
ePercentage of predicted protein sequence covered by matched peptides