Table 2.

Eimeria protein amino acid sequence comparison

Spot IDa	Accession number	Amino acid sequence identity (%)b			SecP/SP/TM predictionc
		E. acervulina	E. tenella	E. necatrixd
1	XP_013248731.1	100	58.72	44.88	0.053/N/N
2	XP_013246990.1	100	51.65	52.07	0.656*/N/N
3	XP_013250980.1	100	38.95	41.18	0.068/N/N
4	XP_013248237.1	100	48.54	48.71	0.371/N/N
5	XP_013252320.1	100	75.00	–	0.533/N/N
6	XP_013250573.1	100	75.19	75.19	0.843*/N/N
7	XP_013249139.1	100	95.14	94.33	0.462/N/N
8	XP_013249709.1	100	61.07	62.09	0.798*/N/N
9	XP_013248843.1	100	89.58	85.42	0.435/N/N
10	XP_013248247.1	100	73.67	73.35	0.42/N/N
11	XP_013248554.1	100	50.00	51.80	0.662*/N/N
12	XP_013251305.1	100	94.10	92.63	0.635*/N/N
13	XP_013251976.1	100	66.54	66.73	0.404/N/N
14	XP_013247421.1	100	58.44	60.60	0.245/N/N
15	XP_013251081.1	100	80.95	81.28	0.172/N/N
16	XP_013248652.1	100	84.56	89.52	0.799*/N/N
17	XP_013253345.1	100	96.00	96.00	0.283/N/N
18	XP_013248150.1	100	61.90	62.67	0.423/N/N
19	XP_013250395.1	100	61.68	47.85	0.298/N/N
20	XP_013251340.1	100	58.02	59.36	0.376/N/N
21	XP_013252670.1	100	67.26	67.51	0.454/N/N

^a2-dimensional gel electrophoresis (2-DE) gel spot number

^bAll protein sequences within the same row are compared to that of E. acervulina using CLUSTAL Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/)

^cNon-classical secretory property (SecP) and presence of a signal peptide (SP) and transmembrane domains (TM) were analyzed by the SecretomeP 2.0a Server (http://www.cbs.dtu.dk/services/SecretomeP/), SignalP-5.0 Server (http://www.cbs.dtu.dk/services/SignalP/) and TMHMM Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/), respectively

^dEimeria necatrix ortholog for XP_013252320.1 was not found

Abbreviations: *, a SecP score exceeds the threshold (0.6), indicative of a secretory potential through the non-classical pathway for proteins with no signal peptides; N, not present