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. 2020 Feb 21;16:66. doi: 10.1186/s12917-020-02288-5

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Lumpy skin disease virus, observed clinical signs and molecular (PCR) confirmation results: Cattle showing characteristic LSDV clinical signs; nodular skin lesions covering the whole body; and lacrimal discharge (panel A shows a cow with nodular skin lesions covering the whole body, panel B shows skin nodules on the neck and fore body and panel C shows skin nodules covering the whole body and lacrimal discharge). Panel D; PCR results showing a 192 bp fragment of the LSDV P32 gene, Lane M is a 100 bp molecular ladder (GeneDireX Inc., UK), lane N is a negative control, lane P a positive control. Lane 2 is a negative sample, while lanes 1 and 3 are samples positive for LSDV. All PCR products were run in 1.5% agarose gel