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. 2020 Feb 18;13:1481–1496. doi: 10.2147/OTT.S229055

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© 2020 Zhang et al.

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Effect of DEPDC1B knockdown on the migration and invasion of PC cells in vitro. (A, B) The DEPDC1B interference efficiency of transient transfection with siRNAs in PC cells was examined by Western blotting and qRT-PCR. (C) Typical images of the wound healing assay for the siNC and siDEPDC1B#1 groups show cell migration to the wound; the images were captured at 0 and 24 h after wounding. (D) Typical images of the transwell migration assay (without Matrigel) and (E) transwell invasion assay (with Matrigel) showing migrated and invaded cells on the membrane. The average migrated and invaded cell numbers from five random visual fields were calculated from three independent experiments. (F) EMT-related protein levels were analyzed in the siNC group and siDEPDC1B#1 group by Western blotting. *P <0.05, **P <0.01, and ***P <0.001.

Abbreviations: DEPDC1B, DEP domain-containing protein 1B; EMT, epithelial-mesenchymal transition; PC, pancreatic cancer; qRT-PCR, quantitative real-time PCR.