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. 2020 Feb 18;13:1481–1496. doi: 10.2147/OTT.S229055

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© 2020 Zhang et al.

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Effect of DEPDC1B overexpression on the migration and invasion of PC cells in vitro. (A, B) DEPDC1B overexpression efficiency in PC cells after stable transfection with LV-CON319 and LV-DEPDC1B was examined by Western blotting and qRT-PCR. (C) Typical images of the wound healing assay of the LV-CON319 and LV-DEPDC1B groups show cell migration to the wound; the images were captured at 0 and 24 after wounding. (D) Typical images of the transwell migration assay (without Matrigel) and (E) transwell invasion assay (with Matrigel) showing the migrated and invaded cells on the membrane. The average migrated and invaded cell numbers from five random visual fields were determined from three independent experiments. (F) EMT-related protein levels were analyzed in the LV-CON319 and LV-DEPDC1B groups by Western blotting. *P <0.05, **P <0.01, and ***P <0.001.

Abbreviations: DEPDC1B, DEP domain-containing protein 1B; EMT, epithelial-mesenchymal transition; PC, pancreatic cancer; qRT-PCR, quantitative real-time PCR.