Figure 7.

Effect of the Rac1 inhibitor EHop-016 on DEPDC1B-induced migration and invasion in PC cells in vitro. (A) Typical images of the wound healing assay in the LVCON319, LV-DEPDC1B and EHop-016 treated LV-DEPDC1B groups show cell migration to the wound; the images were captured at 0 and 24 after wounding. (B) Typical images of transwell migration assays (without Matrigel) and (C) transwell invasion assays (with Matrigel) showing migrated and invaded cells on the membrane. The average migrated and invaded cell numbers from five random visual fields were determined from three independent experiments. (D) EMT-related protein levels were analyzed by Western blotting in the LVCON319, LV-DEPDC1B and EHop-016-treated LV-DEPDC1B groups. *P <0.05, **P <0.01, and ***P <0.001.

Abbreviations: DEPDC1B, DEP domain-containing protein 1B; EMT, epithelial-mesenchymal transition; PC, pancreatic cancer.