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. 2020 Feb 18;12:1221–1227. doi: 10.2147/CMAR.S234891

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© 2020 Ke et al.

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DCST1-AS1 and miR-92a-3p cannot regulate the expression of each other. The potential interaction between DCST1-AS1 and miR-92a-3p was predicted by performing IntaRNA. It was observed that DCST1-AS1 and miR-92a-3p can form strong base pairing between each other (A, left). Dual luciferase reporter assay was performed by co-transfecting HEC-1 cells with either the combination of DCST1-AS1 vector + miR-92a-3p (miR-92a-3p group) or the combination of DCST1-AS1 vector + NC miRNA (NC group). Luciferase activity was measured at 48-hrs post-transfection and was compared (A, right). HEC-1 cells were transfected with DCST1-AS1 vector or miR-92a-3p mimic to further analyze the interaction between them. Overexpression of DCST1-AS1 and miR-92a-3p was confirmed by qPCR at 24-hrs post-transfection (B). The effects of DCST1-AS1 and miR-92a-3p overexpression on the expression of each other were also analyzed by qPCR at 24-hrs post-transfection (C). Experiments were repeated 3 times and mean values were presented. *p<0.05.