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. 2020 Feb 18;13:1507–1518. doi: 10.2147/OTT.S231249

Figure 3.

Figure 3

miR-124 was a target of HNF1A-AS1. (A) The predicted wild-type and mutated type of miR-124 binding sites in the 3ʹUTR of HNF1A-AS1 were exhibited. (B and C) Luciferase activity was measured in SW620 (B) and HCT116 (C) cells co-transfected with HNF1A-AS1-WT or HNF1A-AS1-MUT and miR-124 or miR-NC through luciferase reporter assay. (D and E) RIP assay was performed to ensure the relationship between HNF1A-AS1 and miR-214 in SW620 (D) and HCT116 (E) cells. (F and G) the expression of miR-124 was detected in SW620 (F) and HCT116 (G) cells transfected with pcDNA, HNF1A-AS1, si-NC or si-HNF1A-AS1. ***P < 0.001.