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. 2020 Feb 18;13:1497–1505. doi: 10.2147/OTT.S234920

Figure 1.

Figure 1

Expression of C3 was down-regulated in HSCs via shC3 transfection. (A) Representative western blot bands and quantification of C3a abundances in HSCs (HSC) or treatment with vehicle (shCon) or shRNA after cells generated with puromycin for 1 week. The relative intensity was normalized to actin and relative to the HSC. (B) The C3 mRNA level of HSCs, shCon HSCs, or shC3 HSCs was measured by real-time qPCR cells generated with puromycin for 1 week. The statistical significance was labeled as ***p < 0.001, ns, p > 0.05, n = 3.