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. 2020 Feb 18;7:19–31. doi: 10.2147/JHC.S237614

Figure 1.

Figure 1

PKM2 plays an important role in HCC cells. (A) The expression of PKM2 mRNA was detected by RT-PCR. The data are expressed as the mean ± SD (n = 3, *P < 0.05 vs LO2). (B) Western blotting was used to determine the expression of PKM2 protein. β-actin was used as a loading control. The data are expressed as the mean ± SD (n = 3, *P < 0.05 vs LO2). (C) The expression of PKM2 mRNA in LM3 and SMMC-7721 cells was detected by RT-PCR after PKM2-OE or PKM2-shRNA use. Data are shown as the mean ± SD (n = 3, *P < 0.05 for PKM2-OE vs EV or for PKM2-shRNA vs scramble). (D) Expression of PKM2 protein in LM3 and SMMC-7721 cells was determined by Western blotting after PKM2-OE or PKM2-shRNA use. Data are shown as the mean ± SD (n = 3, *P < 0.05 for PKM2-OE vs EV or for PKM2-shRNA vs scramble). (E) CCK8 was used to detect cell viability after PKM2-OE or PKM2-shRNA use. The data are expressed as the mean ± SD (n = 3, *P < 0.05 for PKM2-OE vs EV; #P < 0.05 for PKM2-shRNA vs scramble). (F and G) Apoptosis of LM3 and SMMC-7721 cells was determined by Hoechst 33342 and flow cytometry after PKM2-OE or PKM2-shRNA use. (H) Expression of Bcl-2, Bax, cleaved-caspase 3, and cleaved-caspase 9 protein was detected by Western blotting. (I) LM3 and SMMC-7721 cells were cultured for 48 h. Glucose uptake and relative lactate production were analyzed. The data are shown as the mean ± SD (n = 3, *P < 0.05 for PKM2-OE vs EV; #P < 0.05 for PKM2-shRNA vs scramble).