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. Author manuscript; available in PMC: 2021 Jan 3.
Published in final edited form as: Science. 2020 Jan 3;367(6473):76–79. doi: 10.1126/science.aax1898

Fig. 3. GS and ES properties of Dronpa2 amber suppression variants.

Fig. 3.

Circled numbers refer to processes depicted in Fig. 1B. (A) FQY versus TE and (B) ES energy barrier height versus TE both exhibit a peaked shape (see fig. S7 for comparable FQY results from GFP variants). Linear fits to the electron-donating and electron-withdrawing substituent data in (B) are shown as dashed lines with positive and negative slope, respectively. (C) Isosteric series of the GS energy barrier height plotted against TE, with a dashed line representing a linear fit. (D) Summary of GS and ES properties of Dronpa2 variants.