TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference(s) or source |
|---|---|---|
| Strains | ||
| C. rodentium | ||
| DBS100 | Wild-type C. rodentium | 59 |
| T307 | DBS100 containing pJB861 | This study |
| T329 | DBS100 ΔtatC::cat | This study |
| T390 | DBS100 containing pZ300 | This study |
| T391 | T329 containing pZ300 | This study |
| T520 | T329 containing pMW118 | This study |
| T521 | T329 containing pMW-tatC | This study |
| T333 | DBS100 containing pACYC-gfp | This study |
| T646 | DBS100 ΔtatC | This study |
| T647 | T646 containing pACYC-gfp | This study |
| T690 | Nalidixic acid-resistant DBS100 containing pJB861 | This study |
| S. Typhimurium | ||
| SL1344 | Wild-type S. Typhimurium | 64, 65 |
| T321 | SL1344 ΔtatC::kan | 23 |
| Plasmids | ||
| pBAD24 | High-level expression vector, Apr | 66 |
| pZ300 | pBAD24 expressing TorA::GFP, Apr | Miki & Hardt |
| pJB861 | Broad-host-range cloning vector, Kmr | 67 |
| pMW118 | Low-copy-no. expression vector, Apr | Nippon Gene |
| pMW-tatC | pMW118 carrying tatC, expressing TatC | This study |
| pACYC-gfp | pACYC184 expressing GFPmut3.1 | 23 |
a
Apr, apramycin resistant; Kmr, kanamycin resistant.