FIG 6.
mRNA expression level of proinflammatory cytokines in avian cells at 6 h postinfection with S. Pullorum (C79-13, ΔpSPI12, ΔpSPI12-pipaJ) or S. Enteritidis (P125109, P125109-pipaJ, P125109-pBR322) evaluated using TaqMan qRT-PCR. The expression levels of proinflammatory cytokines were measured in HD-11 cells infected with S. Pullorum (A) and S. Enteritidis (B). The corrected threshold cycle (CT) values are shown as fold change in the mRNA level of cytokines in comparison to those from uninfected controls. Asterisks indicate differences between levels of cytokines induced by different strains, *, P < 0.05; **, P < 0.01; ***, P < 0.001. mRNA expression level of proinflammatory cytokines in chMDM cells infected with S. Pullorum (C) and S. Enteritidis (D). chMDM cells were primary blood monocyte-derived macrophages isolated from chicken blood.