TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| C79-13 | Wild-type S. Pullorum strain (WT) | Chinese Institute of Veterinary Drug Control |
| ΔpSPI12 | pSPI12 plasmid mutant strain of C79-13 | 26 |
| ΔpSPI12-pipaJ | ΔpSPI12 complemented with expression of IpaJ from pBR322 | 26 |
| P125109 | Wild-type S. Enteritidis strain | Laboratory stock |
| P125109-pipaJ | P125109 complemented by pBR322-ipaJ | 26 |
| P125109-pBR322 | P125109 transformed with the pBR322 plasmid | 26 |
| ΔSPI1 | S. Pullorum strain S06004 with SPI1 deleted | 39 |
| ΔSPI2 | S. Pullorum strain S06004 with SPI2 deleted | 39 |
| ΔSPI1 SPI2 | S. Pullorum strain S06004 with both SPI1 and SPI2 deleted | 39 |
| ΔSPI1 pipaJ-HA | ΔSPI1 complemented with pBR322-ipaJ-HA | This work |
| ΔSPI2 pipaJ-HA | ΔSPI2 complemented with pBR322-ipaJ-HA | This work |
| ΔSPI1 SPI2 pipaJ-HA | ΔSPI1 SPI2 complemented with pBR322-ipaJ-HA | This work |
| ΔpSPI12 pipaJ-HA | ΔpSPI12 complemented with pBR322-ipaJ-HA | This work |
| SNI | S. flexneri strain | Laboratory stock |
| Plasmids | ||
| pCMV-HA | Mammalian expression vector expressing proteins containing an N-terminal hemagglutinin (HA) epitope tag | Laboratory stock |
| pCMV-HA-ipaJ | Vector expressing IpaJ from S. Pullorum | This work |
| pCMV-HA-ipaJ(C90A) | Vector expressing IpaJ replacing cysteine at position 90 with alanine | This work |
| pCMV-HA-ipaJ(H230A) | Vector expressing IpaJ replacing histidine at position 230 with alanine | This work |
| pCMV-HA-ipaJ(D242A) | Vector expressing IpaJ replacing aspartic acid at position 242 with alanine | This work |
| pCMV-HA-FipaJ | Vector expressing IpaJ from S. flexneri | This work |
| pBR322 | Cloning vector | TaKaRa |
| pBR322-ipaJ | Cloning vector expressing IpaJ | 26 |
| pBR322-ipaJ-HA | Cloning vector expressing IpaJ with HA tag | This work |