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. 2020 Feb 20;88(3):e00690-19. doi: 10.1128/IAI.00690-19

FIG 2.

FIG 2

Characterization of selenocystine-resistant tcyA::Tn mutant isolates. (A) Representative tcyA::Tn selenocystine-resistant colonies were isolated and tested for their resistance in the presence of 100 mM selenocystine. The first number for each bar indicates the concentration of selenocystine (50 mM, 75 mM, or 100 mM) added via the Whatman disk to the plate the mutants first appeared, and the second number represents the isolate number from that concentration of selenocystine. The dotted line represents the disk diameter (6 mm). (B) Growth of tcyA::Tn selenocystine-resistant mutants in 25 μM cystine. Data represent the mean of at least three independent trials. Error bars represent ± 1 standard error of the mean.