FIG 4.
TcyABC and TcyP support proliferation in medium supplemented with cysteine (Cys) or N-acetyl-cysteine (NAC) as the sole sulfur source. WT, tcyA::Tn, tcyP::Tn, and tcyAP::Tn strains were cultured anaerobically in PN medium supplemented with 100 mM sodium nitrate to induce anaerobic respiration and 25 μM cystine (A), 50 μM cysteine (B), or 50 μM N-acetyl-cysteine (C) as the sole sulfur source. The mean of at least three independent trials is presented. Error bars represent ± 1 standard error of the mean.