FIG 6.
Expression of tcyP and tcyA is induced in sulfur starvation conditions. The comparative ΔΔCT methodology was used to calculate fold change and rho was used as a reference gene. Log2 fold change of tcyA and tcyP transcripts relative to T0 was determined. S. aureus was cultured in PN supplemented with 25 μM CSSC and then transferred to medium containing 25 μM CSSC or no sulfur source (starved). Bars represent the mean of at least three independent trials. The error bars represent ± 1 standard error of the mean. * indicates P < 0.05 determined from Student's t test.