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TcyP is required for maximal fitness in the murine heart and liver. BALB/c mice were systemically infected with a 1:1 ratio WT and the indicated mutant strain in Newman (A and B) or JE2 (C and D) strains of S. aureus. Competitive indices were calculated as the output ratio of mutant to WT CFU ml−1 over the input mutant to WT CFU ml−1. The mean competitive index for liver (A and C) and heart (B and D) is presented. Error bars represent ± 1 standard error of the mean.
