FIG 12.

Fluorescence microscopy, using DAPI and fluorescence in situ hybridization (FISH), of sections of control and infected tissues showing bacterial invasion. (A and B) DAPI experiments showing the anterior region of the nematode inserted in the intestinal tissue and bacilliform bacteria (arrows) close to the nematode cuticle. In panel B, it is possible to identify the bacteria (arrows) and the bacillary band of the T. muris nematode. (C and D) FISH experiments showing the three layers of the uninfected cecum tissue and the bacteria (green) present only in the mucosa. (E and F) FISH experiments showing infected tissue invaded by bacteria (green) in the submucosa (arrows). (D and F) Merged figures showing bacteria (green) and host tissue (DAPI). Bars, 10 μm (A and B) and 50 μm (C to F). The images are representative of those from 5 experiments. Abbreviations: N, nematode; c, cuticle; Bb, bacillary band; Mu, mucosa; Sm, submucosa; M, muscularis.