FIG 2.
MHC-I and MHC-II are involved in antibody isotype switching. B2m KO, MHC-II KO, and WT C57BL/6 mice were vaccinated s.c. with 10 μg of PIV and challenged i.p. with 1 × 107 genomic copies of C. burnetii NMI 28 dpv. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. C. burnetii NMI-specific serum IgM (A to C) and IgG (D to F) were evaluated weekly following vaccination. Specific IgM (G) and IgG (H) were also evaluated 14 dpi. Each experimental group includes five mice, with error bars representing the standard deviations from the mean. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (as determined by two-way ANOVA with Sidak’s multiple-comparison test [A to F] or t test [G and H]).