Figure 3. MgSO4 downregulates IL-1β mRNA expression from HUVECs.
HUVECs were stimulated with LPS (LPS 10, 100 ng/ml), BzATP (10 μM, A) or (100 μM, B), BBG (100 μM, C), and with or without MgSO4 (0.1, 1, 10, 100 mM, D) for 3-hrs. LPS±BzATP induced IL-1β mRNA expression which was inhibited by MgSO4 similar to the effect of BBG. Values are expressed as mean±SEM. p<0.0001 one-way ANOVA with Tukey’s multiple comparisons test.
| Fig 3a. | Mean (Fold Change) | SEM | n |
|---|---|---|---|
| Control | 1 | 0 | 3 |
| LPS100 | 76.63316 | 10.7092 | 3 |
| LPS100+MgSO4100 | 0.9004739 | 0.2190636 | 3 |
| LPS100+BBG100 | 42.80225 | 11.72334 | 3 |
| LPS100+BzATP10 | 111.2056 | 10.80206 | 3 |
| LPS100+BzATP10+MgSO4100 | 0.05896 | 0.03743 | 3 |
| LPS100+BzATP10+BBG100 | 5.92331 | 3.761908 | 3 |
| Fig 3b. | |||
| Control | 1 | 0 | 3 |
| LPS10 | 23.28879 | 6.860098 | 3 |
| LPS100 | 76.63316 | 10.7092 | 3 |
| BzATP100 | 0 | 0 | 3 |
| LPS100+BzATP100 | 87.5097 | 13.39503 | 3 |
| Fig 3c. | |||
| Control | 1 | 0 | 3 |
| LPS100 | 76.63316 | 10.7092 | 3 |
| LPS100+BBG100 | 42.80225 | 11.72334 | 3 |
| LPS100+BzATP100 | 87.5097 | 13.39503 | 3 |
| LPS100+BzATP100+BBG100 | 15.52824 | 2.632421 | 3 |
| Fig 3d. | |||
| Control | 1 | 0 | 3 |
| LPS100 | 76.63316 | 10.7092 | 3 |
| LPS100+MgSO40.1 | 62.12084 | 14.59779 | 3 |
| LPS100+MgSO41 | 62.61264 | 4.782178 | 3 |
| LPS100+MgSO410 | 50.23698 | 10.24755 | 3 |
| LPS100+MgSO4100 | 1.958895 | 0.8618461 | 3 |
| LPS100+BzATP100 | 87.5097 | 13.39503 | 3 |
| LPS100+BzATP100+MgSO4100 | 2.927146 | 1.023443 | 3 |