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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Nanotoxicology. 2019 Sep 25;14(2):145–161. doi: 10.1080/17435390.2019.1668067

Figure 6: Glutathione Redox Potential Measurement in Rat Aortic Endothelial Cells Exposed to ZnO Nanoparticles and Charge Transfer.

Figure 6:

RAECs were exposed to pristine, annealed, oxidized or reduced ZnO NPs for 24hrs at 20 μg/ml. Intracellular reduced and oxidized glutathione (GSH (A) and GSSG (B) respectively) concentrations were measured using HPLC (N=3). (C) The redox potential (Eh) was calculated using the Nernst equation (Eh=E0 + (RT/nF)ln([acceptor]/[donor]). (D) Measurement of ZnO NPs direct reactivity with cell free GSH incubated for 15 min at 37 °C as measured by Elman’s reagent. (E) ZnO NP cyclic voltammetry for the measurement of charge transfer between gluthathione and ZnO NPs. A three-electrode setup measured the charge transfer of ZnO (1 mg/ml) nanoparticles with and without chemical defects to gluthathione (1mM). *statistically significant compared to control (untreated cells). #statistically significant compared to pristine ZnO NPs (p<0.05).