Skip to main content
. Author manuscript; available in PMC: 2021 Feb 20.
Published in final edited form as: Mol Cell. 2019 Dec 31;77(4):810–824.e8. doi: 10.1016/j.molcel.2019.12.003

Fig. 2. MUFAs selectively activate SIRT1.

Fig. 2.

A) Saturation plot of SIRT1 activity towards FOXO3a and the effects of 18:1 and resveratrol (n=4). B) Lineweaver-Burk reciprocal plots were generated to determine Km, Vmax, and Kcat for the FOXO3a peptide substrate. C-D) Km and Kcat/Km fold change for each concentration of 18:1 on FOXO3a. E) Kcat/Km fold change for resveratrol (Res; 10 μM). F) Saturation plot of SIRT1 activity towards H3 and the effects of 18:1 and resveratrol. G) Lineweaver-Burk reciprocal plots for the H3 peptide substrate. H-I) Km and Kcat/Km fold change for each concentration of 18:1 with H3. J-K) Kcat/Km fold change for each concentration of resveratrol and fatty acids for the H3 peptide substrate. L) Competition assay of SIRT1 activity towards FOXO3A, PGC-1α and H3 acetylated peptide substrates.