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. Author manuscript; available in PMC: 2020 Jun 16.
Published in final edited form as: Nat Chem Biol. 2019 Dec 16;16(3):310–317. doi: 10.1038/s41589-019-0423-2

Figure 4. Measuring FRET-efficiency for the intermediate FRET state during CHL-induced translational arrest.

Figure 4.

a. Schematic diagram of smFRET assay using Cy3B–Cy5 pair to measure FRET efficiencies of non-rotated, rotated and intermediate FRET states of the ribosome during translation in the presence of CHL. b. Representative FRET trace and state assignments for the ribosome translating MFKAFK mRNA in the presence of 1 μM CHL (similar results observed for n = 94). c. FRET-efficiency histogram for each state (+,x,◊) and fitted normal distributions (line).