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. Author manuscript; available in PMC: 2020 Jun 16.
Published in final edited form as: Nat Chem Biol. 2019 Dec 16;16(3):310–317. doi: 10.1038/s41589-019-0423-2

Figure 5. The glycyl residue of the A-site tRNA rescues CHL-arrested ribosomes.

Figure 5.

a. Schematic diagram of experiments using Cy5-labeled tRNALys mischarged with Gly (Gly-(Cy5)-tRNALys). b. Representative fluorescence traces of translation of the MFKAFK mRNA in the reaction containing the mischarged Gly-(Cy5)-tRNALys in the absence (top) or presence (bottom) of CHL (similar results observed for n = 134 and 120 for top and bottom plots, respectively). c. Ribosome survival plots showing that the presence of Gly-tRNALys promotes bypassing of the CHL-induced translation arrest at the F5 codon of the MFKAFK template (n = 94, 134 and 120 from left to right).