Fig. 1.

The level of TUG1 in the lung tissues of septic mice and the effect of TUG1 overexpression in sepsis-induced ALI. Adult C57BL/6 mice were randomly assigned into 4 groups: sham, CLP, CLP + Ad-GFP, and CLP + Ad-TUG1 (n = 12 per group). CLP, CLP + Ad-GFP, CLP + Ad-TUG1 groups received intravenous injections of vehicle, control adenoviral vector, and adenoviral vector expressing TUG1, respectively, followed by the CLP operation. Animals in the sham group underwent the same CLP procedure without the ligation or puncture of the cecum. (a) The expression of TUG1 in mouse lung tissues were examined using qRT-PCR. (b) Survival rate of mice within 72 h following CLP procedure. (c) Paraffin-embedded lung tissue samples were stained for H&E and the degree of lung damage was determined using lung injury scoring. Representative histological images were shown at 400× magnification. (d) The serum level of TUG1 in ARDS patients (n = 35) and healthy subjects (n = 68) was measured using a ELISA kit