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. 2020 Feb 22;20:49. doi: 10.1186/s12890-020-1084-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Impact of TUG1 upregulation on CLP-induced apoptosis and inflammation in mouse lung tissues. (a) Histological analysis of sectioned mouse lung tissue samples using TUNEL staining and immunohistochemistry for caspase-3 expression. Representative histological images (400× magnification) and the percentage of positively stained cells were shown. (b) The levels of Bax, Bcl-2, and cleaved PARP in the lung tissues of all mice were analyzed using Western blot. Representative plots (left) and bar charts (right) were shown. (c-d) The mRNA and proteins expressions of TNF-α, IL-1β and IL-6 in all animals were detected using qRT-PCR and ELISA, respectively. (e) The protein expressions of IL-4 and IL-10 in mouse lung tissues were measured using ELISA