Table 2.

Summary of all RT-PCR primers and PCR protocols used

Gene	Forward primer sequence (5′ → 3′)	Reverse primer sequence (5′ → 3′)	PCR protocol	Cycles
FLT3	TTTCACAGGACTTGGACAGAGATTT	GAGTCCGGGTGTATCTGAACTTCT	95 °C/50 °C/72 °C	40
VIM	GAACTTTGCCGTTGAAGCTG	TCTCAATGTCAAGGGCCATC	95 °C/56 °C/73 °C	40
CDH1	GTCAGGTGCCTGAGAACG	GCCATCGTTGTTCACTGG	95 °C/56 °C/73 °C	40
POLR1D (V1)	CCACCTGAGGATCCAGAAAC	CCTCGTGCAATACAAATGTCA	95 °C/60 °C	40
POLR1D (V2)	AAGAACTGCTTAAGGAGGCAA	TCTTCGCTGGTTCCTTATCG	95 °C/60 °C	40
PAN3	TTGGTGCCCTCAACATCTCT	TTGATCCCATCGGAACTAGC	95 °C/60 °C	40
CDX2	GAACCTGTGCGAGTGGATG	TCCTCCGGATGGTGATGTAG	95 °C/60 °C	40
PDX1	CCTTTCCCATGGATGAAGTC	TTCAACATGACAGCCAGCTC	95 °C/60 °C	40
LNX2	ATGCAACGTTGTGATCTGGA	CCAAACAGTCTGCTTCTGGA	95 °C/60 °C	40
GAPDH	CCAAAATCAAGTGGGGCGATG	AAAGGTGGAGGAGTGGGTGTCG	95 °C/60 °C	40