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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: J Vis Exp. 2019 Nov 12;(153):10.3791/60440. doi: 10.3791/60440

Figure 1: Scheme for preparation of mouse embryonic motor neurons.

Figure 1:

The schematic illustrates the steps involved in the isolation and culture of mouse embryonic motor neurons and the approximate time in hours or days for each step. The order of the dissection procedure for CN3/CN4 and for SMN are each labelled sequentially 1 through 4. Abbreviations: CN3/CN4: oculomotor neuron/trochlear neuron; SMN: spinal motor neuron; FACS: fluorescence-activated cell sorting; h: hour; d: day.