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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: J Vis Exp. 2019 Nov 12;(153):10.3791/60440. doi: 10.3791/60440

Figure 4: Phase-contrast images of primary CN3/CN4 and SMN monocultures at 2, 7, and 14 DIV.

Figure 4:

Representative differential interference contrast images of primary CN3/CN4 and SMN cultures were captured at 2, 7, and 14 DIV with inverted fluorescence microscope using corresponding image acquisition and processing software and 40× objectives. Neuronal processes become thicker and longer by 14 DIV. Neuronal cell body sizes become enlarged and tend to aggregate in long-term cultures, especially for SMNs. Both cultures can be maintained at least 14 DIV. Scale bar: 50 μm.