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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: J Vis Exp. 2019 Nov 12;(153):10.3791/60440. doi: 10.3791/60440

Figure 6: Representative immunocytochemistry of primary CN3/CN4 and SMN monocultures at 2, 7, and 14 DIV.

Figure 6:

Primary CN3/CN4 and SMN cultures were analyzed at 2, 7, 14 DIV by immunofluorescence labeling with TUJ1 (green) and nuclei were counterstained with DAPI (blue). Neuronal processes become thicker and longer by 14 DIV. Neuronal cell body sizes become enlarged and tend to aggregate in long-term cultures, particularly for SMNs. Both CN3/CN4 and SMN cultures can be maintained at least 14 DIV. Images were captured under 10× magnification. Scale bar: 200 μm.