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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: J Vis Exp. 2019 Nov 12;(153):10.3791/60440. doi: 10.3791/60440

Figure 8: Representative application of primary motor neuron culture demonstrating that CN3s/CN4s are selectively resistant to ER stress induced by CPA.

Figure 8:

A) Experimental outline: primary CN3/CN4 and SMN monocultures were treated with CPA or vehicle control (DMSO) at 2 DIV and cell viabilities were evaluated through immunocytochemistry analysis after 3 days of treatment. This outline has been modified from published work31. B) Quantification of survival ratios of CN3s/CN4s and SMNs treated with 5–30 μM CPA for 3 days from 2 DIV. Neurons were analyzed by immunofluorescent labeling of cells with TUJ1, and nuclei were counterstained with DAPI. Survival ratios were calculated as the number of viable cells (refer to Figure 5B legend) in drug-treated wells divided by the number of viable cells in wells containing vehicle alone (DMSO). Cell counting was performed manually under 20× magnification. Values represent the mean ± SEM of 4 separate experiments. *P < 0.05; ***P < 0.005 by Student’s t test. This figure has been modified from published work31.