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. 2020 Feb 20;477(4):763–772. doi: 10.1042/BCJ20190832

Figure 3. α-catenin involve in the negative feedback mechanism during glucose stimulated insulin secretion.

Figure 3.

INS-1E cells were transfected with either control siRNA or siRNA specific for α-E catenin or α-N catenin. Forty-eight hours after siRNA transfection, cells were serum starved for 2 h and stimulated with either 0.5 mM Glucose or 10 mM glucose for 2 h. Insulin concentrations were measured by performing alphaLIZA assays and were normalised to total protein content. (A) Immunoblot analysis of α-E catenin levels in INS-1E lysates after siRNA transfection and (B) insulin concentrations in supernatants of negative control or α-E catenin siRNA transfected cells. (C) Immunoblot analysis of α-N catenin protein level in INS-1E lysates after siRNA transfection and (D) insulin concentrations in supernatants of negative control or α-N catenin siRNA transfected cells. Results are mean ± S.E.M. * P < 0.05 and ** P < 0.01 compared with control glucose stimulated condition as assessed by two-way ANOVA. Similar results were obtained in at least three independent experiments.